Illumina

Created

January 1, 2026

Modified

March 29, 2026

Sequencing Basics

Understanding the fundamentals of Illumina sequencing including clusters, flowcells, reads, lanes, indexing, quality scores, and file formats.

Illumina Sequencing Libraries

Detailed information about Illumina library structures including TruSeq Single Index, TruSeq Dual Index, and Nextera Dual Index libraries, plus the library sequencing workflow.

Illumina 5-base

Overview of Illumina’s 5-base methylation detection workflow.

---
title: Illumina
date: 2026-01-01
published-title: Created
date-modified: last-modified
title-block-banner: "#212529"
---

## [Sequencing Basics](sequencing-basics.qmd)

Understanding the fundamentals of Illumina sequencing including clusters, flowcells, reads, lanes, indexing, quality scores, and file formats.

## [Illumina Sequencing Libraries](sequencing-libraries.qmd)

Detailed information about Illumina library structures including TruSeq Single Index, TruSeq Dual Index, and Nextera Dual Index libraries, plus the library sequencing workflow.

## [Illumina 5-base](illumina-5-base.qmd)

Overview of Illumina's 5-base methylation detection workflow.