- Map: Align reads to the reference.
- Call: Decide if a “T” is a 5th base (5mC) or a mutation.
- Overlay: Compare those sites to a database of known genes and CpG islands.
- Compare: Look for differences between samples to find “Biological Hits.”
January 1, 2026
March 29, 2026
---
title: "Illumina 5-base"
date: 2026-01-01
published-title: Created
date-modified: last-modified
title-block-banner: "#212529"
toc: true
toc-location: left
format:
html:
css: illumina.css
---
1. Map: Align reads to the reference.
2. Call: Decide if a "T" is a 5th base (5mC) or a mutation.
3. Overlay: Compare those sites to a database of known genes and CpG islands.
4. Compare: Look for differences between samples to find "Biological Hits."